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作者

Zeqin Li, Na Liu, Fang Yuan, Zimeng Guan, Jinding Liu, Feng Liu, Jianbo Ren, Jiangwei Yan, Gengqian Zhang

作者单位

Affiliations ¹ School of Forensic Medicine, Shanxi Medical University, Jinzhong, 030600, Shanxi, China. ² Shanxi Key Laboratory of Forensic Medicine, Jinzhong, 030600, Shanxi, China. ³ Department of Biotechnology, Biomedical Sciences College, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250117, Shandong, China. ⁴ School of Forensic Medicine, Shanxi Medical University, Jinzhong, 030600, Shanxi, China. yanjw@sxmu.edu.cn. ⁵ Shanxi Key Laboratory of Forensic Medicine, Jinzhong, 030600, Shanxi, China. yanjw@sxmu.edu.cn. ⁶ School of Forensic Medicine, Shanxi Medical University, Jinzhong, 030600, Shanxi, China. gengqianzhang@sxmu.edu.cn. ⁷ Shanxi Key Laboratory of Forensic Medicine, Jinzhong, 030600, Shanxi, China. gengqianzhang@sxmu.edu.cn.

刊名

International journal of legal medicine

年份

2024

卷号

Vol.138 No.3

页码

1205-1219

ISSN

1437-1596

关键词

Blood identification DNA methylation Mixed stains SNP SNaPshot.

摘要

Blood-containing mixtures often appear in murder and robbery cases, and their identification plays a significant role in solving crimes. In recent years, the co-detection of DNA methylation markers and single nucleotide polymorphism markers has been shown to be a promising tool for the identification of semen and its donor. However, similar research on blood stains that are frequently found at crime scenes has not yet been reported. In this study, we employed blood-specific CpG-linked SNP mark...更多

Blood-containing mixtures often appear in murder and robbery cases, and their identification plays a significant role in solving crimes. In recent years, the co-detection of DNA methylation markers and single nucleotide polymorphism markers has been shown to be a promising tool for the identification of semen and its donor. However, similar research on blood stains that are frequently found at crime scenes has not yet been reported. In this study, we employed blood-specific CpG-linked SNP markers for blood-specific genotyping and the linking of blood and its donor. The tissue-specific CpG markers were screened from the literature and further verified by combining bisulfite conversion with amplification-refractory mutation system technology. Meanwhile, adjacent SNP markers with a minor allele frequency greater than 0.1 were selected within 400 bp upstream and downstream of the CpG markers. SNP genotyping was performed using SNaPshot technology on a capillary electrophoresis platform. Finally, a multiplex panel, including 19 blood-specific CpG linked to 23 SNP markers, as well as 1 semen-specific CpG, 1 vaginal secretion-specific CpG, and 1 saliva-specific CpG marker, was constructed successfully. The panel showed good tissue specificity and blood stains stored at room temperature for up to nine months and moderately degraded could be effectively identified. Moreover, it could also be detected when blood content in the mixed stains was as low as 1%. In addition, 15 ng of DNA used for bisulfite conversion was required for obtaining a complete profile. The cumulative discrimination power of the panel among the Han population of northern China could reach 0.999983. This is the first investigation conducted for the simultaneous identification of blood and its donor regardless of other body fluids included in mixed stains. The successful construction of the panel will play a vital role in the comprehensive analysis of blood-containing mixtures in forensic practice.收起

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