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作者

Lina Zhang Lu Zhang Dandan Liu Shujing Wang Ruifang Liu Longfei Zhu Chengxiang Li Li Xie Shenguang Ge Juan Wang

作者单位

⁴Shandong Provincial Key Laboratory of Precision Oncology, Cancer Research Center, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China ²These authors contributed equally to this work. ⁵Department of Otorhinolaryngology, Qilu Hospital of Shandong University, NHC Key Laboratory of Otorhinolaryngology (Shandong University), Jinan, Shandong 250012, China ³Institute for Advanced Interdisciplinary Research(iAIR), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China ¹Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, China

刊名

Sensors and Actuators B: Chemical

年份

2025

卷号

Vol.436

页码

137723

ISSN

0925-4005

摘要

Herein, a target-induced polarity switching photoelectrochemical biosensor was constructed by integrating the exponential amplification reaction with the CRISPR/Cas12a system to achieve more accurate detection of miRNA-622. Based on the n-type semiconductor CN modified electrode to provide initial anodic photocurrent signal, target miRNA-622 triggered the exponential amplification reaction, which converted trace amounts of miRNA-622 into a large quantity of DNA1 and DNA2 molecular. The DNA1 in...更多

Herein, a target-induced polarity switching photoelectrochemical biosensor was constructed by integrating the exponential amplification reaction with the CRISPR/Cas12a system to achieve more accurate detection of miRNA-622. Based on the n-type semiconductor CN modified electrode to provide initial anodic photocurrent signal, target miRNA-622 triggered the exponential amplification reaction, which converted trace amounts of miRNA-622 into a large quantity of DNA1 and DNA2 molecular. The DNA1 introduced the p-type semiconductor BiOBr nanosheets binding the DNA tetrahedron modified electrode surface to convert the PEC signal into a cathodic photocurrent. In the meantime, the DNA2 as activators bound with crRNA and Cas12a to form the ternary complex and activated the trans-cutting characteristics of Cas12a, cleaving H1-loaded BiOBr and leading to the quench of the cathodic photocurrent. Benefiting from the PEC polarity switching and EXPAR assisted CRISPR-Cas12a system, the proposed sensing platform exhibited high sensitivity with an excellent detection limit of 0.92 fM . Polarity transformation of PEC biosensor possessed the superiority of effectually avoiding the issue of false positive/negative signals in the detection process as a result of PEC signal with more accurate analysis achieved, which is promising to be applied for cancer diagnosis and treatment monitoring.收起

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