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作者

Huijuan Li Huijun Li Shuang Qi Xiaohui Tang Shiqi Zhao Xin Bian Baoqing Tian Hua Zhang Yuchun Wei Dianlong Jia Xinyue Han Qing Fan

作者单位

³Public Health Clinical Center Affiliated to Shandong University/Shandong Public Health Clinical Center, Jinan 250100, PR China ²Shandong Center for Food and Drug Evaruation and Inspection, Jinan 250014, PR China ⁴State Key Laboratory of Macromolecular Drugs and Large-scale Preparation, School of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252059, PR China ¹Shandong Cancer Hospital and Institute, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan 250117, PR China

刊名

International Journal of Pharmaceutics: X

年份

2025

卷号

Vol.10

页码

100413

ISSN

2590-1567

关键词

Skin cancer EGFR Affibody Tumor diagnosis Photothermal therapy

摘要

The epidermal growth factor receptor is a critical therapeutic target implicated in the pathogenesis and progression of skin cancer, holding significant promise for enhancing precision diagnosis and treatment efficacy. In this study, we developed ICG-Z, a novel EGFR-targeting agent engineered through conjugation of the photosensitizer indocyanine green to an EGFR-targeted dimeric affibody for the theranostics of EGFR-positive skin cancer. In vitro, ICG-Z-mediated photothermal therapy induced...更多

The epidermal growth factor receptor is a critical therapeutic target implicated in the pathogenesis and progression of skin cancer, holding significant promise for enhancing precision diagnosis and treatment efficacy. In this study, we developed ICG-Z, a novel EGFR-targeting agent engineered through conjugation of the photosensitizer indocyanine green to an EGFR-targeted dimeric affibody for the theranostics of EGFR-positive skin cancer. In vitro, ICG-Z-mediated photothermal therapy induced significant cell death in EGFR-positive A431 cells, while exhibiting minimal effects on EGFR-negative MLE-12 cells. Compared to free ICG, ICG-Z enhanced tumor retention, and significantly improved tumor-targeting capability, making it advantageous for identifying EGFR-positive tumor tissues. Furthermore, ICG-Z also exhibited excellent photothermal conversion performance in vivo, and effectively suppressed the growth of A431 tumors through thermal ablation. Importantly, ICG-Z demonstrated favorable short-term safety profiles during the in vivo treatment assay. In conclusion, the successfully developed ICG-Z in this study, as a novel tumor-targeted photosensitizer, innovatively breaks through the limitations of traditional single-modal treatment. This study would open up new perspectives and pathways for integrated clinical diagnosis and treatment of EGFR-positive skin cancer.收起

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期刊