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作者

Qingyu Huang, Fuhao Wang, Xiaohui Li, Wenxue Zou, Zhou Huang, Xia Li, Wenhui Yang, Tianyu Lei, Shengqin Yue, Qian Wang, Qi An, Bicheng Zhang, Cong Wang, Chao Liu, Qinyong Hu

作者单位

Affiliations ¹ Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ² Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117, China. ³ Department of Medical Oncology, Peking University First Hospital, Beijing, 100034, China. ⁴ Department of Oncology, Linyi Center Hospital, Linyi, Shandong, China. ⁵ Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Radiation Oncology, Peking University Cancer Hospital & Institute, Beijing, 100142, China. ⁶ Department of Obstetrics and Gynecology, Heze Municipal Hospital, Heze, China. ⁷ Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. bichengzhang@hotmail.com. ⁸ Department of Gynecologic Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117, China. wangcongsdu@16 com. ⁹ Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117, China. charles.liu@whu.edu.cn. ¹⁰ Department of Radiation Oncology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310000, Zhejiang, China. 2925829838@qq.com.

刊名

Cancer immunology, immunotherapy : CII

年份

2025

卷号

Vol.75 No.1

页码

26

ISSN

1432-0851

关键词

B cell Cervical cancer Multi-omics Single-cell RNA sequencing Tertiary lymphoid structure.

摘要

Background: Cervical cancer is a leading cause of cancer-related deaths in women, and understanding the tumor immune microenvironment is crucial for identifying novel biomarkers and therapeutic targets. While T cells have been extensively studied in oncology, the role of B cells in CC remains poorly understood. Methods: In this study, we generated and integrated multi-omics data, including single-cell RNA sequencing, single-nucleus RNA sequencing, spatial transcriptomics, bulk RNA sequencing, a...更多

Background: Cervical cancer is a leading cause of cancer-related deaths in women, and understanding the tumor immune microenvironment is crucial for identifying novel biomarkers and therapeutic targets. While T cells have been extensively studied in oncology, the role of B cells in CC remains poorly understood. Methods: In this study, we generated and integrated multi-omics data, including single-cell RNA sequencing, single-nucleus RNA sequencing, spatial transcriptomics, bulk RNA sequencing, and multiplex immunofluorescence to investigate the composition and transcriptomic states of B cells in CC. Results: Initially, we analyzed plasma cells, classifying them into IgA and IgG subtypes. We found that the proportion of IgA plasma cells was lower in cancer samples, whereas IgG plasma cells were higher. IgA plasma cells exhibited protein folding and B cell activation characteristics, while IgG plasma cells were associated with apoptosis and immune suppression. Subsequently, we focused on two specific plasma cell populations, MANF_PC and HSPA1B_PC. MANF_PC, which were predominantly enriched in normal tissues, were linked to enhanced antibody synthesis and better prognosis, while HSPA1B_PC, primarily found in cancerous tissues, were associated with apoptotic pathways and poor clinical outcomes. Importantly, HSPA1B_PC are associated with tertiary lymphoid structures , where they show significant spatial colocalization with immunosuppressive T cell subsets, including Tregs and Th17 cells within TLS. Conclusions: We identified distinct plasma cell subsets in CC and found that HSPA1B_PC are enriched in tumors, associated with immunosuppressive T cells in TLS. These findings suggest that HSPA1B_PC contribute to an immunosuppressive microenvironment in CC, highlighting their potential as therapeutic targets.收起

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