获取途径

作者

Yang, X.-H. Peng, L. Han, X.-Y. Chen, D.-Y.

作者单位

Department of Oral and Maxillofacial Surgery, School of Stomatology, Taishan Medical University, Taian, Shandong Province 271000, China a Department of Stomatology, Yeda Hospital of Yantai, Yantai, Shandong Province 264006, China

刊名

Chinese Journal of Tissue Engineering Research

年份

2017

卷号

Vol.21 No.4

页码

545-550

ISSN

1673-8225

关键词

Blood Vessel Prosthesis Endothelial Cells Stem cells Tissue Engineering

摘要

BACKGROUND: Insufficient blood supply after major bone defects leads to osteocyte necrosis; therefore, the tissue-engineered bone cannot meet the clinical requirements. OBJECTIVE: To construct an artificial vessel using in vitro cultured rabbit bone marrow stromal stem cells and human umbilical vein endothelial cells combined with the rabbit aortic decellularized vascular matrix. METHODS: Rabbit bone marrow stromal stem cells were cultured by adherent method, human umbilical vein endothelial cel...更多

BACKGROUND: Insufficient blood supply after major bone defects leads to osteocyte necrosis; therefore, the tissue-engineered bone cannot meet the clinical requirements. OBJECTIVE: To construct an artificial vessel using in vitro cultured rabbit bone marrow stromal stem cells and human umbilical vein endothelial cells combined with the rabbit aortic decellularized vascular matrix. METHODS: Rabbit bone marrow stromal stem cells were cultured by adherent method, human umbilical vein endothelial cells were cultured in vitro and isolated, and rabbit aortic decellularized vascular stent was prepared. The stent-cell composite was constructed, and its biocompatibility was observed under scanning electron microscope. There were three groups: blank control, control and experimental groups , followed by implantation around the small vessels. Three months later, the vascular samples were collected for gross observation and hematoxylin-eosin staining. RESULTS AND CONCLUSION: Fibroblast-like cell clones were formed by adherent method, and were positive for CD44 + and STRO-1 + after climbing; calcified nodule formed in the ossification. Flow cytometry showed CD34 + accounting for 43.83%, and visible VIII factor in the endothelial cells. In the experimental group, endothelial cells formed a single cell layer onto the scaffold under scanning electron microscope; the artificial vessel grew well and tubular structure presented and eight angiod structures were found. Thin film-like vessels formed in the blank control group and there were only endothelial-like tissues in the control group. These results suggest that angiod structures can form after the stent-cell composite implantation, causing no immune reaction, but the artificial vessel is visible in absence of muscular layer.收起

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