Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection

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作者: Mo-Yuan Shen, Jie-Fu Chen, Chun-Hao Luo, Sangjun Lee, Cheng-Hsuan Li, Yung-Ling Yang, Yu-Han Tsai, Bo-Cheng Ho, Li-Rong Bao, Tien-Jung Lee, Yu Jen Jan, Ya-Zhen Zhu, Shirley Cheng, Felix Y Feng, Peilin Chen, Shuang Hou, Vatche Agopian, Yu-Sheng Hsiao, Hsian-Rong Tseng, Edwin M Posadas, Hsiao-Hua Yu

作者单位: Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan. Smart Organic Material Laboratory, Institute of Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Rd., Nankang, Taipei, 11529, Taiwan. Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Los Angeles, CA, 90048, USA. Department of Molecular and Medical Pharmacology, California NanoSystems Institute, University of California, Los Angeles, 570 Westwood Plaza, Los Angeles, CA, 90095-1770, USA. Liver Transplantation and Hepatobiliary Surgery, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA. Departments of Radiation Oncology, Urology, and Medicine, University of California, San Francisco, San Francisco, CA, 94158, USA. Affiliations Department of Material Engineering, Ming Chi University of Technology, 84 Gungjuan Rd., Taishan Dist., New Taipei City, 24301, Taiwan.

关键词: RNA biomarkers circulating tumor cells poly(3,4-ethylene-dioxythiophene)s prostate cancer responsive materials.

摘要: A glycan-stimulated and polys -based nanomaterial platform is fabricated to purify circulating tumor cells from blood samples of prostate cancer patients. This new platform, phenylboronic acid -grafted PEDOT NanoVelcro, combines the 3D PEDOT nanosubstrate, which greatly enhances CTC capturing efficiency, with a poly interfacial layer, which not only provides high specificity for CTC capture upon antibody conjugation but also enables competitive binding of sorbitol to gently release the capture...更多; A glycan-stimulated and polys -based nanomaterial platform is fabricated to purify circulating tumor cells from blood samples of prostate cancer patients. This new platform, phenylboronic acid -grafted PEDOT NanoVelcro, combines the 3D PEDOT nanosubstrate, which greatly enhances CTC capturing efficiency, with a poly interfacial layer, which not only provides high specificity for CTC capture upon antibody conjugation but also enables competitive binding of sorbitol to gently release the captured cells. CTCs purified by this PEDOT NanoVelcro chip provide well-preserved RNA transcripts for the analysis of the expression level of several PCa-specific RNA biomarkers, which may provide clinical insights into the disease.收起

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