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作者

Shi, Y.-Z., Wei, L.-H., Ge, L., Du, H., Song, W.-G.Email Author View Correspondence

作者单位

Department of Histology and Embryology, Taishan Medical College, Shandong Taian 271000, China

刊名

Acta Anatomica Sinica

年份

2009

卷号

Vol.40 No.4

页码

647-650

ISSN

0529-1356

关键词

EMTREE drug terms luteinizing hormone receptor vasculotropin EMTREE medical terms animal cell article controlled study estrus image analysis immunohistochemistry implantation luteal cell mouse nonhuman ovary pregnancy protein expression protein localization stroma cell theca cell Species Index Mus

摘要

Objective: To explore the biological effects of the luteinizing hormone receptor and vascular endothelial growth factor on the ovary of mice during peri-implantation. Methods: The immunohistochemistry SABC method and image analysis were used to study the distribution and changes of the LHR and VEGF in Kunming mouse ovary during estrous, pregnancy of day 1, day 4 and day 6 stage. Results: The expression of LHR-immunoreactive substance and that of VEGF-immunoreactive substance had the same dist...更多

Objective: To explore the biological effects of the luteinizing hormone receptor and vascular endothelial growth factor on the ovary of mice during peri-implantation. Methods: The immunohistochemistry SABC method and image analysis were used to study the distribution and changes of the LHR and VEGF in Kunming mouse ovary during estrous, pregnancy of day 1, day 4 and day 6 stage. Results: The expression of LHR-immunoreactive substance and that of VEGF-immunoreactive substance had the same distribution and changes. Compared with other groups, the level of LHR-immunoreactive substance and that of VEGF-immunoreactive substance increased highly on the stroma cells around larger growing follicles in estrous group . Along with the pregnancy, the positive immunostaining for LHR and VEGF increased gradually on the granulosa lutein cells, and reached the highest level on day 6 of pregnancy. Positive immunostaining for LHR or VEGF on some endothelia and blood cells were observed in day 1 of pregnancy or estrous group respectively. Form day 1 of pregnancy, the theca cells had positive immunostaining for LHR. Conclusion: The expression of LHR and VEGF is closely related with the process of follicle growing, ovulation and corpus luteum formation.收起

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