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作者

E Airaksinen U Gripenberg

作者单位

¹山东第一医科大学附属省立医院神经外科，济南 25002

刊名

Shandong Medical Journal

年份

2022

卷号

Vol.62 No.27

页码

41-45

ISSN

1002-266X

关键词

biological function analysis fibronectin1 functional state of tumor cells glioma immune cell infiltration 免疫细胞浸润 生物学功能分析 纤维连接蛋白1 肿瘤细胞功能状态 胶质瘤 biological function analysis fibronectin1 functional state of tumor cells glioma immune cell infiltration

摘要

Objective To observe the expression changes of fibronectin1gene in glioma tissues and to inves‐ tigate its correlations with clinicopathological features and prognosis of patients. Methods Based on the transcriptome data of gliomas and normal brain tissues in TCGA database and GTEx database, the difference of FN1 gene expression be‐ tween gliomas and normal brain tissues and the correlation between FN1 gene expression and clinicopathological character‐ istics were analyzed. Kaplan-Meier survival...更多

Objective To observe the expression changes of fibronectin1gene in glioma tissues and to inves‐ tigate its correlations with clinicopathological features and prognosis of patients. Methods Based on the transcriptome data of gliomas and normal brain tissues in TCGA database and GTEx database, the difference of FN1 gene expression be‐ tween gliomas and normal brain tissues and the correlation between FN1 gene expression and clinicopathological character‐ istics were analyzed. Kaplan-Meier survival analysis and ROC curve analysis were used to evaluate the prognostic value of FN1 gene in patients with glioma. Tumor immune cell infiltration was analyzed by ssGSEA algorithm and TIMER data‐ base. ClusterProfiler package was used to analyze KEGG pathway and GO functional enrichment. The FN1 protein interac‐ tion network was obtained by STRING database. The correlation between FN1 gene expression and tumor cell function was analyzed by cancerSEA database. Results FN1 gene was significantly up-regulated in gliomas. The high ex‐ pression of FN1 gene was related to increased tumor grade, IDH classification, 1p19q deletion, age and histopathological classification of glioma. High expression of FN1 gene was a poor prognostic factor for patients with glioma. FN1 gene expression was associated with B cells, CD4+ T cells, CD8+ T cells, dendritic cells, neutrophils, macrophages and other immune cells immune infiltration in tumor microenvironment. The expression of FN1 gene waspositively correlated with proliferation of tumor vascular endothelial cells and tumor cell differentiation, and was negatively correlated with tumor cell DNA damage and cell cycle. There were 351 biological processes related to FN1 gene, including complement activation, 28 cellular components, including immunoglobulin complexes, and 94 molecular functions, including antigen binding, etc. There were 29 KEGG molecular signaling pathways, mainly including cytokine-cytokine receptor interaction. The main interacting proteins of FN1 were ITGB1, ITGB3, ITGA3 and so on. Conclusions The expression of FN1 gene in glioma tissues was up-regulated, which was an indicator of poor prognosis；it was related to tumor immune cell infiltra‐ tion and glioma cell function；the biological function of FN1 gene was related to complement activation, immunoglobulin complex, antigen binding and so on；cytokine-cytokine receptor interactions were involved in signal pathways, and the main interaction proteins were ITGB and so on.收起

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