获取途径

作者

Lina Zhang Zuhao Zhang Yang Zheng Shujing Wang Liu Ruifang Longfei Zhu Chengxiang Li Li Xie Shenguang Ge Jinxiang Wu

作者单位

⁶Department of Pulmonary and Critical Care Medicine, Qilu Hospital, Shandong University, Jinan, China High School), Jining, Shandong 272000, China ⁴Jining Jizhou Senior High School (Rencheng Campus of Jining No ²These authors contributed equally to this work. ⁵Shandong Provincial Key Laboratory of Radiation Oncology, Cancer Research Center, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China ³Institute for Advanced Interdisciplinary Research(iAIR), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China ¹Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, China

刊名

Sensors and Actuators B: Chemical

年份

2025

卷号

Vol.426

页码

137140

ISSN

0925-4005

摘要

Electrochemiluminescence has historically faced challenges such as limited ECL emitters and inadequate signal amplification, significantly hindering its practical applications. The development of novel ECL emitters is a crucial factor in advancing high-performance ECL biosensors. In this study, we propose an approach for compositing highly crystalline CN nanoflowers with CN quantum dots to improve the performance of ECL biosensors. The incorporation of highly crystalline CN nanomaterials sign...更多

Electrochemiluminescence has historically faced challenges such as limited ECL emitters and inadequate signal amplification, significantly hindering its practical applications. The development of novel ECL emitters is a crucial factor in advancing high-performance ECL biosensors. In this study, we propose an approach for compositing highly crystalline CN nanoflowers with CN quantum dots to improve the performance of ECL biosensors. The incorporation of highly crystalline CN nanomaterials significantly enhanced the ECL signal. Ferrocene -labelled DNA tetrahedral nanostructures were self-assembled on glassy carbon electrode surface modified with CN NFs@CN QDs. Using microRNA-320d as a model target, the target initiated rolling circle amplification and catalytic hairpin assembly to produce abundant DNA chains, which acted as activators. These activators subsequently bound to crRNA to activate the trans-cleavage activity of the Cas12a system, resulting in the cleavage of the Fc signal molecule located at the vertices of the DTNs. This cleavage caused Fc to dissociate from the electrode surface, thereby weakening the ECL signal. By integrating cascade nucleic acid amplification with CRISPR/Cas12a for signal amplification, the developed ECL biosensor displayed high sensitivity, selectivity, satisfactory analytical performance, with a linear range of 1 fM - 10 nM and a detection limit of 0.342 fM = 3). These results highlight its potential for rapid and sensitive nucleic acid detection.收起

文献类型

期刊

浏览量

5