获取途径

作者

Chengxiang Li Shujing Wang Longfei Zhu Kaiyue Liu Liyuan Jing Li Xie Shenguang Ge Jinghua Yu

作者单位

²These authors contributed equally to this work. ³Shandong Provincial Key Laboratory of Radiation Oncology, Cancer Research Center, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, 250117, China ¹Institute for Advanced Interdisciplinary Research (iAIR), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

刊名

Analytica Chimica Acta

年份

2025

卷号

Vol.1379

页码

344713

ISSN

0003-2670

摘要

Background MicroRNAs , as pivotal biomarkers, demonstrate critical significance in early cancer diagnosis through their sensitive detection. An ultrasensitive photoelectrochemical sensing platform for miRNA-320d detection was developed by integrating apurinic/apyrimidinic endonuclease 1 enzyme-driven bipedal DNA walker amplification strategy. Results The platform employed a CsBiBr QDs/BiOBr Z-scheme heterojunction as the photoactive material, which generated a robust anodic photocurrent. Upon ...更多

Background MicroRNAs , as pivotal biomarkers, demonstrate critical significance in early cancer diagnosis through their sensitive detection. An ultrasensitive photoelectrochemical sensing platform for miRNA-320d detection was developed by integrating apurinic/apyrimidinic endonuclease 1 enzyme-driven bipedal DNA walker amplification strategy. Results The platform employed a CsBiBr QDs/BiOBr Z-scheme heterojunction as the photoactive material, which generated a robust anodic photocurrent. Upon immobilizing alkaline phosphatase -conjugated gold nanoparticles carrying apurine/pyrimidine site-modified L1 probes on the heterojunction surface, catalytic hairpin assembly -generated 3D bipedal DNA walker walked in the presence of miRNA-320d, hybridizing with L1 to form duplex structures. The APE1 enzyme then selectively cleaved these duplexes, triggering the release of ALP-Au NPs from the electrode surface. This spatial separation deactivated the catalytic capacity of ALP, inducing a pronounced photocurrent attenuation. By synergizing the exceptional PEC performance of the CsBiBr QDs/BiOBr heterojunction, specific recognition and efficient cleavage of APE1 enzyme, and 3D walker-mediated signal amplification, this platform achieved ultrasensitive miRNA-320d detection with a detection limit of 0.1 fM and a linear range spanning 1 fM to 1 nM. Significance This study established novel conceptual frameworks for implementing emerging perovskite materials in PEC biosensing platforms targeting microRNA detection.收起

文献类型

期刊

浏览量

2