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作者

Chao Liu, Yanjuan Chen, Xiaohui Li, Zhijie Bai, Meilin Jiang, Dongsheng Sheng, Wenxue Zou, Rui Huang, Qingyu Huang, Fuhao Wang, Jingyang Zhu, Huiru Sun, Bing Liu, Zongcheng Li, Bing Sun

作者单位

Affiliations ¹ Department of Radiation Oncology, Peking University First Hospital, Beijing, 100034, China. ² Department of Geriatrics and Division of Rheumatology and Research, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, 518020, China. ³ Department of Medical Oncology, Peking University First Hospital, Beijing, 100034, China. ⁴ State Key Laboratory of Experimental Hematology, Institute of Hematology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100071, China. ⁵ Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, 510632, Guangdong, China. ⁶ Department of Thoracic Surgery, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100071, China. ⁷ Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117, China. ⁸ Department of Radiation Oncology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100071, China. ⁹ State Key Laboratory of Experimental Hematology, Institute of Hematology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100071, China. bingliu17@yahoo.com. ¹⁰ State Key Laboratory of Experimental Hematology, Institute of Hematology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100071, China. lizc07@vip.qq.com. ¹¹ Department of Radiation Oncology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100071, China. sunice116@16 com.

刊名

Cancer immunology, immunotherapy : CII

年份

2025

卷号

Vol.74 No.3

页码

80

ISSN

1432-0851

关键词

Immune response Immunotherapy Non-small-cell lung cancer SABR Single-cell RNA sequencing.

摘要

Background: Stereotactic ablative radiotherapy is thought to activate T cell responses in patients with cancer, leading to its combination with immunotherapy and chemotherapy for treatment of non-small-cell lung cancer . Here, we aimed to provide a high-resolution transcriptomic profiling of the systemic T cell response following SABR, with or without preceding immunotherapy/chemotherapy. Methods: We conducted single-cell RNA and T cell receptor sequencing of T cells from peripheral blood of s...更多

Background: Stereotactic ablative radiotherapy is thought to activate T cell responses in patients with cancer, leading to its combination with immunotherapy and chemotherapy for treatment of non-small-cell lung cancer . Here, we aimed to provide a high-resolution transcriptomic profiling of the systemic T cell response following SABR, with or without preceding immunotherapy/chemotherapy. Methods: We conducted single-cell RNA and T cell receptor sequencing of T cells from peripheral blood of seven patients with early-stage NSCLC taken pre- and post-SABR without or with prior immunotherapy and chemotherapy . Other flow cytometry, single-cell RNA-seq data and bulk RNA-seq data were used to validate the results. Results: We uncovered distinct T cell response patterns induced by these treatments: while terminal effector CD8 T cells showed increased cytotoxic and inhibitory scores, and upregulated immune-activated pathways post-SABR, the reverse responses occurred post-icSABR. Furthermore, the proportion of large T cell clones increased and single clone decreased post-SABR, while the opposite was seen post-icSABR. Of note, both SABR and icSABR largely changed TCR clonotypes, which were mainly large clones post-SABR but single clone post-icSABR, and predominantly from terminal effector CD8 T cells and T helper cells, respectively. Conclusions: These findings reveal a complex interplay between SABR and immunotherapy, with potentially valuable implications for treatment strategies involving SABR and immunotherapy to induce systemic T cell responses for tumor eradication in patients with NSCLC.收起

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