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作者

Feng Zhang Lianwan Chen Jinfu Wen Fahui Li Guohua Song

作者单位

¹The Second Affiliated Hospital, and School of Clinical and Basic Medical Sciences of Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong 250000, China ³Key Laboratory of Biomacromolecules (CAS), CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China ²These authors contributed equally: Feng Zhang, Lianwan Chen.

刊名

Chemical Engineering Journal

年份

2025

卷号

Vol.510

页码

161854

ISSN

1385-8947

摘要

Electron microscopy offers high-resolution imaging of cellular ultrastructure, including membranes, organelles, and other nanoscale features. Current genetically encoded tags for catalyzing 3,3′-diaminobenzidine polymerization, however, face limitations due to their bulky size, which often compromises the native localization of target proteins. To address this, we introduce a compact, genetically engineered variant of horse heart myoglobin as a novel DAB-polymerizing tag for labeling proteins...更多

Electron microscopy offers high-resolution imaging of cellular ultrastructure, including membranes, organelles, and other nanoscale features. Current genetically encoded tags for catalyzing 3,3′-diaminobenzidine polymerization, however, face limitations due to their bulky size, which often compromises the native localization of target proteins. To address this, we introduce a compact, genetically engineered variant of horse heart myoglobin as a novel DAB-polymerizing tag for labeling proteins in mammalian cells. Owing to its significantly smaller molecular weight, the HM fusion protein achieves superior localization accuracy compared to the widely used APEX2 system. Furthermore, HM exhibits exceptional resilience under stringent EM fixation protocols, maintaining both ultrastructural integrity and protein-specific labeling fidelity. These attributes position HM as a robust tool for high-resolution EM studies, uncovering the structure and physiological functions of key eukaryotic proteins.收起

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