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作者

Wang, Haiming Zhang, Zhenjie Chen, Zhanqiang Zhang, Yanru Lv, Qiang An, Xiaoping Tong, Yigang Carr, Michael J. Sun, Shuhong Shi, Weifeng

作者单位

National Virus Reference Laboratory, University College Dublin, Dublin, Ireland Dongying Forest and Plant Protection Station, Dongying, Shandong 257091, China College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271018, China Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China Institute of Pathogen Biology, Taishan Medical College, Tai'an, Shandong 271000, China

刊名

Infection, Genetics & Evolution

年份

2016

卷号

Vol.39

页码

325-329

ISSN

1567-1348

关键词

Avian influenza virus East Asian–Australian migration flyway Genetic diversity H9N2 Reassortment

摘要

To understand the molecular epidemiology and evolution of avian influenza viruses along the East Asian–Australian migration flyway, we collected faecal samples between November 2014 and March 2015 from poultry, environmental sources and wild birds in Dongying, Shandong province and Yancheng, Jiangsu province in eastern China. The presence of AIV RNA was evaluated by real-time PCR and the positivity rate ranged from 0 to 29.3%. In both Dongying and Yancheng, samples collected from live poultry ...更多

To understand the molecular epidemiology and evolution of avian influenza viruses along the East Asian–Australian migration flyway, we collected faecal samples between November 2014 and March 2015 from poultry, environmental sources and wild birds in Dongying, Shandong province and Yancheng, Jiangsu province in eastern China. The presence of AIV RNA was evaluated by real-time PCR and the positivity rate ranged from 0 to 29.3%. In both Dongying and Yancheng, samples collected from live poultry markets had the highest positivity rate for AIV RNA. AIV whole genomes were generated and phylogenetically analysed. Our results demonstrate that most of the viruses belonged to the H9N2 subtype, and could be classified into nine novel genotypes based on the phylogenetic analysis of the eight gene segments of the AIV genomes. This revealed a high genetic diversity of H9N2 in this region and suggested that they might have undergone frequent genetic reassortment. In addition, the internal genes of two viruses from wild birds and several viruses from poultry belonged to the same gene constellation, suggesting a potential inter-host transmission of AIV between wild birds and poultry in live markets along routes of migratory flyways. Our results highlight the high genetic diversity of AIV along the East Asian–Australian migration flyway and the need for more extensive AIV surveillance in eastern China.收起

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