获取途径

作者

Yang, D. Wang, X. Wu, Y. Lu, B. Yuan, A. Leon, C. Guo, N.

作者单位

Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China Department of Pharmacy, China-Japan Friendship Hospital, Beijing, 100029, China Biomedical Engineering School, Carlos University, Avda Universidad 30, Leganes, Madrid 28911, Spain a Zhong Yuan Academy of Biological Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, 67 Dong Chang Xi Lu, Liaocheng, 252000, China Metabolomics, Genome Center, UC Davis, 451 Health Sciences Drive, Davis, CA 95616, United States

刊名

Molecules

年份

2015

卷号

Vol.20 No.7

页码

11915-11929

ISSN

1420-3049

关键词

Chronic heart failure GC/TOF-MS Metabolomic pathway analysis Metabolomics Shenfu decoction

摘要

Shenfu decoction can be used to treat patients with sign of Yangqi decline or Yang exhaustion related to chronic heart failure . We conducted a gas chromatography with time-of-flight mass spectrometer -based metabolomic study to increase the understanding of CHF and assess the efficacies and mechanisms of SFD in treating CHF induced by coronary artery ligation in rats. Based on unsupervised principal component analysis, there was a clear separation between the CHF and sham surgery group, which ...更多

Shenfu decoction can be used to treat patients with sign of Yangqi decline or Yang exhaustion related to chronic heart failure . We conducted a gas chromatography with time-of-flight mass spectrometer -based metabolomic study to increase the understanding of CHF and assess the efficacies and mechanisms of SFD in treating CHF induced by coronary artery ligation in rats. Based on unsupervised principal component analysis, there was a clear separation between the CHF and sham surgery group, which revealed that CHF disturbed the metabolism of endogenous substances and significantly altered the urine metabolite fingerprints. After SFD treatment, the metabolomics profile found in CHF was significantly reversed, shifting much closer to normal controls and sham surgery group, indicating that SFD has therapeutic effects in CHF, which is in accordance with the hemodynamic assay results. Metabolomic pathway analysis demonstrated that several pathways including fatty acid biosynthesis, fatty acid elongation, steroid biosynthesis, galactose metabolism, and amino acid metabolism were significantly altered in CHF rats. Therefore, we may infer that SFD shows therapeutic efficacy in CHF by restoring these disturbed metabolic pathways, especially those related to energy metabolism. This study offers new methodologies for increasing the understanding of CHF and systematically characterizing the efficacies and mechanisms of SFD in treating CHF.收起

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