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作者

Huansheng Wang Fajun Pei Hao Li Bing Li Shili Han Huajie Yu Sheng Li

作者单位

Cheeloo College of Medicine，Shandong University，Jinan，China；Shandong Cancer Hospital and Institute，Shandong First Medical University and Shandong Academy of Medical Sciences，Jinan，China；Shandong Cancer Hospital and Institute，Shandong First Medical University and Shandong Academy of Medical Sciences，Jinan，China；Shandong Pharmaceutical Research Institute，Jinan，China；Shandong Pharmaceutical Research Institute，Jinan，China；Wuhan YZY Medical Science and Technology Co.，Wuhan，China；Department of Anesthesiology，Zhangqiu District People’s Hospital，Jinan，China；Cheeloo College of Medicine，Shandong University，Jinan，China；Shandong First Medical University，Jinan，China

刊名

转化医学年鉴

年份

2022

卷号

第10卷

期号

第2期

页码

50

关键词

Fluorescent probe circulating tumor cell (CTC) immunocytochemistry (ICC) identification

摘要

Background To develop a novel highly accurate circulating tumor cell identification method and to validate its application in cancer diagnostics and/or prognostics. Methods We verified and validated the combined fluorescent probe staining protocol through CTC and non-CTC morphological comparison of five tumor cell lines in vitro and 32 patient tumor samples from the Shandong Cancer Hospital and Institute. Wright’s Giemsa staining and cluster differentiation 45 immunocytochemistry staining ...更多

Background To develop a novel highly accurate circulating tumor cell identification method and to validate its application in cancer diagnostics and/or prognostics. Methods We verified and validated the combined fluorescent probe staining protocol through CTC and non-CTC morphological comparison of five tumor cell lines in vitro and 32 patient tumor samples from the Shandong Cancer Hospital and Institute. Wright’s Giemsa staining and cluster differentiation 45 immunocytochemistry staining were used as reference control methods. The association between the developed method and clinicopathology was also investigated. Results We successfully developed and optimized the protocol, and validated the use of combined fluorescent probe staining for the identification of CTCs in the peripheral blood of tumor cell lines and tumor patients. Comparable CTC and non-CTC morphologies were observed for combined fluorescent probe staining and Giemsa staining methods in vitro. However, in vivo comparison between the three staining methods revealed that the identified CTCs differed in cell diameter and nucleo-cytoplasmic ratio. In addition, a higher CTC detection rate of 14/32, lower standard deviation , and higher area under the receiver operating characteristic curve value of 0.844 were noted for combined fluorescence staining. Clinicopathological analysis revealed that CTCs were correlated with platelet levels , but not with age, gender, drinking history, or granule ratio. Conclusions We developed a combined fluorescent probe staining method with higher CTC identification accuracy than Wright’s Giemsa staining, and propose this technique as a novel clinical diagnostic/prognostic tool.收起

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