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作者单位

² 山东省肿瘤医院 (山东省肿瘤防治研究院) 肝胆外科, 山东济南25011 ⁴ 山东第一医科大学第二附属医院普外科, 山东泰安27100 ³ 山东省肿瘤医院 (山东省肿瘤防治研究院) 麻醉科, 山东济南25011 ¹ 山东第一医科大学 (山东省医学科学院) 临床与基础医学院, 山东济南25011

刊名

Shandong Medical Journal

年份

2025

卷号

Vol.65 No.1

页码

34-37

ISSN

1002-266X

关键词

liver cirrhosis primary liver carcinoma Raman spectroscopy surgical margin 原发性肝癌 手术切缘 拉曼光谱技术 肝硬化

摘要

Objective To investigate the efficacy of Raman spectroscopy in the identification of primary liver cancer and liver cirrhosis, and to provide a novel approach for determining the safe surgical margin distance in liver cancer surgeries. Methods Thirty samples of liver cancer tissues and 30 cases of para-cancerous cirrhotic tissues were collected from patients with primary liver cancer complicated by liver cirrhosis. Raman spectroscopy analysis was performed using a microscopic confocal Raman spec...更多

Objective To investigate the efficacy of Raman spectroscopy in the identification of primary liver cancer and liver cirrhosis, and to provide a novel approach for determining the safe surgical margin distance in liver cancer surgeries. Methods Thirty samples of liver cancer tissues and 30 cases of para-cancerous cirrhotic tissues were collected from patients with primary liver cancer complicated by liver cirrhosis. Raman spectroscopy analysis was performed using a microscopic confocal Raman spectrometer. The peak intensities of the Raman spectra at various positions in primary liver cancer tissues were compared with those in cirrhotic tissues, and a receiver operating characteristic curve was drawn to evaluate the application value of Raman spectroscopy in distinguishing primary liver cancer and cirrhotic tissues. Results Raman spectrum analysis demonstrated that the intensity of Raman peaks in primary liver cancer tissues was significantly lower than that in cirrhosis tissues at the Raman peaks of 1, 004, 1, 155, and 1, 510 cm⁻¹. In contrast, at the Raman peak of 1, 642 cm⁻¹, the peak intensity for primary liver cancer tissues surpassed that of cirrhosis tissues . Notably, significant characteristic peaks specific to primary liver cancer tissues were identified near the Raman peaks at 1, 377 and 1, 586 cm⁻¹, while these peaks were not present in cirrhosis tissues. The results of ROC curve analysis indicated that the area under the ROC curve of Raman spectroscopy in differentiating primary liver cancer and cirrhosis was equal to 1 at the peaks of 1, 004, 1, 155, 1, 510, and 1, 642 cm⁻¹. The corresponding optimal critical values were 248. 5, 531. 0, 773. 5, and 249. 5 counts, respectively. Both the sensitivity and specificity were 100% . Conclusion Raman spectroscopy can effectively identify liver cancer tissues and para-cancerous cirrhotic liver tissues near the key peaks of 1, 004, 1, 155, 1, 510, and 1, 642 cm⁻¹, and has potential clinical application value.收起

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