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作者

Jingjing Lan Ruihua Zhang Honglei Yu Jingyu Wang Wenxiang Xue Junhao Chen Shaoli Lin Yu Wang Zhijing Xie Shijin Jiang

作者单位

Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian 271000, China College of Public Health and Management, Weifang Medical University, Weifang 261042, China Molecular Virology Laboratory, VA-MD College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA ude.dmu@0991lsl Department of Basic Medical Sciences, Taishan Medical College, Taian 271000, China moc.361@1-4791uygnaw College of Veterinary Medicine, Shandong Agricultural University, Taian 271000, China moc.361@4201naljj (J.L.) moc.621@721140iuriur (R.Z.) moc.361@00850836871 (H.Y.) moc.361@676gnawyj (J.W.) moc.361@37169749051 (W.X.) moc.361@yynehc-oahnuj (J.C.) nc.ude.uads@jhzeix (Z.X.)

刊名

International Journal of Molecular Sciences

年份

2019

卷号

Vol.20 No.24

页码

6160

ISSN

1422-0067

关键词

duck hepatitis A virus type 1 quantitative proteomics differentially expressed proteins host–pathogen interaction ER stress-induced autophagy

摘要

Autophagy is a tightly regulated catabolic process and is activated in cells in response to stress signals. Despite extensive study, the interplay between duck hepatitis A virus type 1 and the autophagy of host cells is not clear. In this study, we applied proteomics analysis to investigate the interaction mechanism between DHAV-1 and duck embryo fibroblast cells. In total, 507 differentially expressed proteins were identified, with 171 upregulated proteins and 336 downregulated proteins. The...更多

Autophagy is a tightly regulated catabolic process and is activated in cells in response to stress signals. Despite extensive study, the interplay between duck hepatitis A virus type 1 and the autophagy of host cells is not clear. In this study, we applied proteomics analysis to investigate the interaction mechanism between DHAV-1 and duck embryo fibroblast cells. In total, 507 differentially expressed proteins were identified, with 171 upregulated proteins and 336 downregulated proteins. The protein expression level of heat shock proteins and their response to stimulus proteins and zinc finger proteins were significantly increased while the same aspects of ribosome proteins declined. Bioinformatics analysis indicated that DEPs were mainly involved in the “response to stimulus”, the “defense response to virus”, and the “phagosome pathway”. Furthermore, Western blot results showed that the conversion of microtubule-associated protein 1 light chain 3-I to the lipidation form of LC3-II increased, and the conversion rate decreased when DEF cells were processed with 4-phenylbutyrate . These findings indicated that DHAV-1 infection could cause endoplasmic reticulum stress-induced autophagy in DEF cells, and that ER stress was an important regulatory factor in the activation of autophagy. Our data provide a new clue regarding the host cell response to DHAV-1 and identify proteins involved in the DHAV-1 infection process or the ER stress-induced autophagy process.收起

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