First characterization of four repeat regions with the bla carried on an IncFII plasmid in Enterobacter hormaechei

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作者单位: ¹State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China ³Department of Structure and Morphology, Jinan Microecological Biomedicine Shandong Laboratory, Jinan, Shandong, China ⁶Research Units of Infectious Diseases and Microecology, Chinese Academy of Medical Sciences, Beijing, China ⁸Lead contact ²Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China ⁷Yuhang Institute for Collaborative Innovation and Translational Research in Life Sciences and Technology, Hanghzou, China ⁴Department of Neurosurgery, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China ⁵Department of Gastrointestinal Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China

摘要: Carbapenem-resistant Enterobacteriaceae have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four bla genes in tandem on an IncFII plasmid from Enterobacter hormaechei. Whole-genome sequencing of E. hormaechei isolates L710hy identified a four-tandem repeat of the bla gene, with each copy’s environment nearly identical -VI-ISAba125-bla-ble-iso-tat-dsbD-cutA-sul1-ISC...更多; Carbapenem-resistant Enterobacteriaceae have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four bla genes in tandem on an IncFII plasmid from Enterobacter hormaechei. Whole-genome sequencing of E. hormaechei isolates L710hy identified a four-tandem repeat of the bla gene, with each copy’s environment nearly identical -VI-ISAba125-bla-ble-iso-tat-dsbD-cutA-sul1-ISCR1). The study suggests a correlation between the bla copy number and minimum inhibitory concentration values for carbapenems, though further research is needed to confirm this correlation. The stability of these four bla tandem repeat structures was also investigated. This is the first documented case of bla tandem repeats in E. hormaechei, and the ISCR1 and other insertion sequence may play a role in this arrangement.收起

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