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作者

Congyi Cheng Chunlin Wang Weichun Zhu Cong Su Youran Dong Junlin Teng Kang Fu Chungang Zhai Lei Qiao Wenqiang Chen

作者单位

Key Laboratory of Cardiovascular Remodeling and Function Research of MOE, NHC, CAMS and Shandong Province, Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China ²Department of Cardiology (Chest Pain Center), Central Hospital Affiliated to Shandong First Medical University, Jinan, China ¹State Key Laboratory for Innovation and Transformation of Luobing Theory

刊名

Frontiers in Cardiovascular Medicine

年份

2025

卷号

Vol.12

关键词

coronary heart disease angiopoietin-like protein 7 coronary collateral circulation severe coronary stenosis clinical prognosis angiogenesis

摘要

BackgroundAngiogenesis and coronary collateral circulation formation promote cardiac repair following severe coronary stenosis or myocardial infarction . Angiopoietin-like protein 7 is a secreted protein associated with angiogenesis, but its role in CCC formation remains unclear.ObjectiveThe aim of this study was to investigate the role of ANGPTL7 in angiogenesis and evaluate the predictive value of serum ANGPTL7 in CCC formation and the prognosis of patients with SCS.Materials and methodsThe...更多

BackgroundAngiogenesis and coronary collateral circulation formation promote cardiac repair following severe coronary stenosis or myocardial infarction . Angiopoietin-like protein 7 is a secreted protein associated with angiogenesis, but its role in CCC formation remains unclear.ObjectiveThe aim of this study was to investigate the role of ANGPTL7 in angiogenesis and evaluate the predictive value of serum ANGPTL7 in CCC formation and the prognosis of patients with SCS.Materials and methodsThe RNA sequencing was performed on myocardial tissues of mice to analyze the alterations of angiogenesis-related genes after MI. 100 patients with angiographically proven SCS and 36 controls were enrolled and retrospectively followed up. Serum ANGPTL7 was measured by enzyme-linked immunosorbent assays . Human umbilical vein endothelial cells and exogenous human recombinant ANGPTL7 protein were applied in assays including CCK-8, scratch, tube formation, cell immunofluorescence and western blot to demonstrate the proangiogenic effect of ANGPTL7. Gene Set Enrichment Analysis was used to perform KEGG pathway enrichment analysis on downstream mechanisms by which ANGPTL7 promoted angiogenesis.ResultsThe transcriptional level of Angptl7 was upregulated in ischemic myocardial tissues of MI mice, and its serum levels increased in both mice post-MI and patients with SCS. Spearman correlation analysis indicated that serum ANGPTL7 levels were positively correlated to CCC grades . Kaplan–Meier curves showed a higher serum ANGPTL7 was associated with a lower incidence of major adverse cardiovascular events in patients with SCS . Cox proportional hazards regression analyses showed that serum ANGPTL7 level was remained a protective factor after adjusting for different covariates. Time-dependent receiver-operating characteristics curves further explored the prognostic value of ANGPTL7, with the area under the curve of 0.77 at 1 year, 0.70 at 2 years and 0.85 at 3 years. Additionally, ANGPTL7 enhanced endothelial cell proliferation, migration and capillary-like structure formation, indicating a proangiogenic effect in vitro.ConclusionANGPTL7 serves as a predictive biomarker for CCC levels and the prognosis of patients with SCS, which probably attributed to its proangiogenic properties.收起

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